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FC 800-C
密閉式螢光成像系統
Closed FluorCam
http://psi.cz/
 Photon Systems Instruments

用途:

   FluorCam FC 800-C 密閉式螢光成像系統是一多光譜段動力學螢光成像系統, 包含CCD 鏡頭、4-5LED 光源板(4+1選購光源)、以及選購的濾片. LED 光源板提供樣品恆定的輻射照度. 樣品室可以進行暗適應. 適用多種樣品,可測量單個、分離葉片或培養皿中培養物以及經稀釋的海藻微小物等.


預設或使用者指定協定:

  • FV/FM
  • Kautsky induction
  • Quenching analysis
  • Steady state fluorescence, e.g., ChlF, GFP and other FPs (filter wheel required)
  • Multicolor fluorescence, e.g., ChlF, plant blue-green fluorescence (filter wheel required)
  • QA re-oxidation (needs optional electronic module)
  • Fast fluorescence induction (OJIP) with 1µs resolution (needs optional electronic module)
  • PAR absorptivity (needs optional accessories: filter wheel and additional IR LED panel)

葉綠素螢光參數:

  • Measured parameters: FO, FM, FV, FO', FM', FV', FT
  • More than 50 calculated parameters: FV/FM, FV'/FM', PhiPSII , NPQ, qN, qP, Rfd, PAR-absorptivity coefficient, electron transport rate (ETR), and many others

應用:

  • Screening for photosynthetic performance and metabolic perturbations
  • Detection of biotic and abiotic stress
  • Plant’s resistance or susceptibility to various stress factors
  • Stomatal patchiness
  • Yield improvement
  • Growth and development

樣品:

  • Leaves, small plants, fruits, vegetables
  • Mosses, lichens
  • Cyanobacteria, green algae
  • Sample size up to 13 cm x 13 cm
  • Selectable shelf system for different plant size
  • Predefined imaging masks for 384-well plate, 96-well plate, Petri dish, etc.
  • Dark room for adaptation

不同放射段:

  • 8 position filter wheel (optional)
  • Chlorophyll fluorescence (high pass 695 nm, low pass 780 nm), GFP (high pass 495, low pass 660 nm, band pass 505/560 nm), PAR (clear glass), YFP, CY3, CY5, as well as other fluorescence colors
  • See also: Closed FluorCam GFP Version for detection and imaging of GFP, red-shifted GFP, or blue-emitting protein variants

標準畫面格式:

  • 512 x 512 pixels
  • A/D: 12 bit (4096 grey levels)
  • 8.2 µm x 8.4 µm pixel size
  • 50 images per second
  • Advantageous for rapid processes measurements

軟體:

  • Fully automated control of the whole FluorCam system
  • Image acquisition through automated experimental protocols:
    - numerous predefined protocols,
    - possibility to create user defined protocols via Windows Wizard,
    - multiple (automatically repeated) experiments,
    - Barcode reader support
  • Image processing tools:
    - automatic or manual image segmentation – e.g., labeling of individual plants,
    - analysis of kinetic data from all samples within the field of view,
    - numerous image manipulation tools,
    - export to text file, avi, bmp or raw data formats
Windows 2000, XP, Vista, W7 compatible                                     

規格:

  • Fluorescence Parameters:
    - Measured parameters: FO, FM, FV, FO', FM', FV', FT
    - More than 50 calculated parameters: FV/FM, FV'/FM', PhiPSII , NPQ, qN, qP, Rfd, PAR-absorptivity coefficient, electron transport rate (ETR), and many others
  • Light Sources:                                                                       - - 455 nm, 470 nm, 505 nm, 570 nm, 605 nm, 618 nm, 630 nm, 735 nm, white and others
  • Super Pulse Intensity:
    - 3,000 µmol(photons)/m².s (in a standard version)
    - 10,000 µmol(photons)/m².s (in the light-upgraded version)
  • Actinic Light Intensity:  Up to 2,500 µmol(photons)/m².s (depending on light wavelength)
  • Filter Wheel:  8 positions
  • Light Regime:  Static or dynamic (sinus form)
  • Custom-Defined Protocols:  Variable timing, special language and scripts
  • CCD Detector Wavelength Range:  400 – 1000 nm
  • CCD Format:  512 x 512 pixels; optionally 1024 x 768 pixels or 1392 x 1040 pixels
  • Pixel Size:  8.2 µm x 8.4 µm; optionally 6.45 µm x 6.45 µm
  • A/D bit Resolution:  12 bit
  • Spectral Response:  QE max at 540 nm (~70 %), 50 % roll-off at 400 nm and 650 nm
  • Read-Out Noise:  Less than 12 electrons RMS - typically only 10 electrons
  • Full-Well Capacity:  Greater than 70,000 electrons (unbinned)
  • Imaging Frequency:  Maximum 50 frames per second
  • Bios:  Upgradeable firmware
  • Communication Port:  USB 2.0
  • Dimension:  471 mm (W) x 473 mm (D) x 512 mm (H)
  • Weight:  Appr. 40 kg
  • Power Input:  Appr. 1100 W
  • Electrical:  90 – 240

      ;(中文綜合目錄下載)