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FC 800-O
開放式螢光成像系統
open-fluorcam
http://psi.cz/
 Photon Systems Instruments

用途:

  FluorCam FC 800-O開放式植物螢光成像系統 是一彈性的模組儀器,用於各種尺寸的樣品(從培養盤到小型植物), 4LED光源板可調整與樣品間的角度與距離. 也可依據植物的高度調整相機的位置.  LED光源板的選擇,最大取樣區尺寸為13 x 13 cm 20 x 20 cm (標準硬體). 選購LED光源板並將系統固定在可移動支架上,最大取樣區為 200 x 100 cm, 請參考 XY-Plane, Transect, Arch, Rover Customized FluorCams, Open FluorCam video.


預設或使用者指定協定:

  • FV/FM
  • Kautsky induction
  • Quenching analysis
  • Steady state fluorescence, e.g., ChlF, GFP and other FPs (filter wheel required)
  • Multicolor fluorescence, e.g., ChlF, plant blue-green fluorescence (filter wheel required)
  • PAR absorptivity (needs optional accessories: filter wheel and additional IR LED panel)

  • Open FluorCam in a Dark Adaptation Box

葉綠素螢光參數:

  • Measured parameters: FO, FM, FV, FO', FM', FV', FT
  • More than 50 calculated parameters: FV/FM, FV'/FM', PhiPSII , NPQ, qN, qP, Rfd, PAR-absorptivity coefficient, electron transport rate (ETR), and many others
  • Images of More Than 50 Calculated Parameters in One Measurement

應用:

  • Screening for photosynthetic performance and metabolic perturbations
  • Detection of biotic and abiotic stress
  • Plant’s resistance or susceptibility to various stress factors
  • Stomatal patchiness
  • Yield improvement
  • Growth and development

樣品:

  • Leaves, whole plants, small trees, fruits, vegetables
  • Mosses, lichens
  • Cyanobacteria, green algae
  • Sample size up to 13 cm x 13 cm or 20 x 20 cm
  • Predefined imaging masks for 384-well plate, 96-well plate, Petri dish, etc.
  • Dark box for adaptation (optional)

不同放射段:

  • 8 position filter wheel (optional)
  • Chlorophyll fluorescence (high pass 695 nm, low pass 780 nm), GFP (high pass 495, low pass 660 nm, band pass 505/560 nm), PAR (clear glass), YFP, CY3, CY5, as well as other fluorescence colors
  • See also: Closed FluorCam GFP Version for detection and imaging of GFP, red-shifted GFP, or blue-emitting protein variants

標準畫面格式:

  • 512 x 512 pixels
  • A/D: 12 bit (4096 grey levels)
  • 8.2 µm x 8.4 µm pixel size
  • 50 images per second
  • Advantageous for rapid processes measurements

  • Progressive scan CCD, 1.4 M pixel sensor
  • A/D: 12 bit (4096 grey levels)
  • 6.45 µm x 6.45 µm pixel size
  • Two operating modes:
    - video (ChlF kinetics measurement),
    - snapshot (long integration times up to minutes for FPs detection)
  • Resolution:
    - 1392 x 1040 pixels – maximum resolution only in snapshot mode,
    - 1024 x 768 pixels (10 images per second) – maximum resolution in video mode
  • Binning option improves sensitivity and provides higher frame rates (both modes):
    - 2x2 - 696 x 520 pixels, 25 images per second,
    - 3x3 – 464 x 344 pixels, 20 images per second,
    - 4x4 - 348 x 256 pixels, 50 images per second
  • Intended for combined measurement of ChlF and detection of weak steady-state fluorescence signals with long integration times or applications where the higher spatial resolution is of highest importance (microscopy)

軟體:

  • Fully automated control of the whole FluorCam system
  • Image acquisition through automated experimental protocols:
    - numerous predefined protocols,
    - possibility to create user defined protocols via a Windows Wizard,
    - multiple (automatically repeated) experiments,
    - Barcode reader support
  • Image processing tools:
    - automatic or manual image segmentation – e.g., labeling of individual plants,
    - analysis of kinetic data from all samples within the field of view,
    - numerous image manipulation tools,
    - export to text file, avi, bmp or raw data formats
  • Windows 2000, XP, Vista, W7 compatible                                                                                              *Windows 2000, Windows XP, Vista, and W7 are trademarks of Microsoft Corporation.

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